CV Dr. Marcelo Garcés

 

 

AFFILIATIONS


Postdoctoral Researcher
Laboratorio de Fisiología y Biología Molecular Vegetal
Instituto de Agroindustria
Facultad de Ciencias Agropecuarias y Forestales
Universidad de La Frontera

 

 

CONTACT


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Phone: +56 (45) 2325699



ACADEMIC DEGREES


2009 Doctor in Sciences, Plant genetic engineering, Universidad de Talca, Chile
2008 Doctor in Sciences, Evolutionary, functional and community ecology. Université de Bordeaux, France
2001 Biochemist, Universidad de Concepción, Chile

 

 

DOCTORAL THESIS


2008 Proteomic study of wood formation in maritime pine. Joint doctoral thesis Université de Bordeaux-Universidad de Talca. Tutor: Christophe Plomion - Raul Herrera.

 

 

POSTDOCTORAL RESEARCH


2011-2013 Proteomics on the drought tolerant family Hymenophyllacea ferns, do they have constitutive or inducible tolerance mechanisms? Fondecyt postdoctorate Nº3120195.


RESEARCH VISITS


October 2015. University of Guelph. Ontario Canada. Department of Plant Agriculture. Dr Lewis Lukens.
2006-2008 INRA UMR BIOGECO, Bordeaux, France. Doctoral thesis. Dr Christophe Plomion.


RESEARCH LINES


• Proteomic and molecular study of plant drought stress
• Molecular study of fatty acid and PUFA production on marine protists
• Plant genetic engineering

 

 

PUBLICATIONS

 

 

1. Physiological and ultrastructural characterization of a drought tolerant filmy fern, Hymenophyllum caudiculatum. Influence of translational regulation and ABA on recovery. Garcés M, Ulloa M, Miranda A, Bravo L. Plant Biology (2017) online 28 November 2017. DOI:10.1111/plb.12660

 

2. Desiccation tolerance of Hymenophyllacea filmy ferns is mediated by constitutive and not inducible cellular mechanisms. Garcés M, Claverol S, Alvear C, Rabert C, Bravo L. Comptes Rendus Biologie Apr; 337(4): 235-43 (2014).

 

3. Wood proteomics in a pine tree: identification of proteins regulated during ontogenesis. Garcés M, Lalanne C, Claverol S, Le Provost G, Barré A, Bosc A, Plomion C and Herrera R. Tree Physiology 34 (11): 1263-1277 (2014).

 

4. Cytotoxic effect of double emulsion(w/o/w) CuSO4-loaded PLA nanoparticles on MKN-45 gastric adenocarcinoma cell line. Montiel-Eulefi E, Jara F, Toro C, Garcés M, Leal P. International Journal of Morphology (2014). 32 (1):61-69.

 

5. The in vitro translocation Cytoplasm / nucleus of the embryonic transcription factor OCT-4 in perivascular cells, proposes to the aorta as a quiescent niche of adult stem cells Herrera J. Montiel E, Glaser T, Garcés M, Leal P, Henning A. International Journal of Morphology 31(4):1430-1438 (2013).



6. Spent coffee grounds: a source of diterpenes and polyphenols. Acevedo F, Rubilar M, Scheuerman E, Cancino B, Uquiche E, Garcés M, Inostroza K, Shene C. Journal of Biobased Materials and Bioenergy Volume 7, Number 3, julio 2013 pp420-428. (2013)

 

7. Molecular and phenotypic profiling from the base to the crown in maritime pine wood-forming tissue. Paiva J, Garcés M, Alves A, Garnier-Géré P, Rodrigues JC, Lalanne C, Porcon S, Le Provost G, da Silva Perez D, Brach J, Frigerio JM, Claverol S, Barré A, Fevereiro P, Plomion C. New Phytologist 178 (2), 283–301(2008).



8. The Chilean Strawberry [Fragaria chiloensis (L.) Duch.]: Genetic Diversity and Structure. Carrasco B, Garcés M, Rojas P, Saud G, Herrera R, Retamales J, Caligari P. J. Amer. Soc. Hort. Sci. 132(4):501–506. (2007)

 

 

OTHERS PUBLICATIONS (Software)

 

 

Protein Purification (http://www.agbooth.com/pp_ajax/index.html?locale=es). Educational software that simulates the purification of proteins from a mixture, translated into Spanish from the English version of Andrew Booth. University of Leeds UK (2013)

 

 

RESEARCH PROJECTS


2017-2019: Extremophytes: The new promising models for discovering stress tolerance mechanisms ans bioactive compounds Adjunt researcher. NEXER (Network for extreme environment research)

 

 

2016-2018: Antarctic thraustochytrids as source of docosahexaenoic acid, bioactive compounds, and genetic data: isolation and molecular characterization. Co-researcher. INACH Project Nº RT_23-16.

 

 

2013-2016: Rehydration proteome of the desiccation tolerant filmy fern Hymenophyllum caudiculatum, translational regulation and drought protective protein species identification. Main Researcher. Fondecyt Initiation into Research Project Nº 111307717.



2011-2013: Proteomics on the drought tolerant family Hymenophyllacea ferns, Do they have constitutive or inducible tolerance mechanisms? Main Researcher. Fondecyt postdoctorate Nº3120195.

 

 

2011-2012: Quantitative proteomic study of the inhibitory effect on the proliferation of gastric cancer cells produced by coffee extracts residues (Coffea arabica). Main Researcher. PIA-UFRO DI10-7006.


2009-2010: HPV detection and typing kit for therapeutic decisions in preneoplastic and neoplastic cervical lesions. Postdoctoral Researcher. INNOVA CHILE Corfo 07CN13PBT-222.



2008-2009: Production of recombinant Taq polimerase for Molecular Biology Applications. Main researcher.Capital Semilla 9- EM-S2.1-492 INNOVA BIO BIO.



2005-2008: Genomics of wood formation and molecular tools for Breeding wood quality in maritime pine. Doctoral student. Génoplante France GNP05013C.

 

 

PRESENTATION AT NATIONAL/INTERNATIONAL CONGRESSES

 

 

1. Proteomic study of Hymenophyllum caudiculatum, a desiccation tolerant filmy fern, treated with ABA (abscisic acid), cycloheximide, a traductional inhibitor or actinomycin (translational inhibitor). Garcés M, Claverol S, Ulloa M, Miranda A, Bravo L. Gordon Research Conference on Salt & Water Stress in Plants. Les Diablerets, Switzeland May 29th-June 3rd, 2016.

 

 

2. Physiological and ultraestructural characterization of Hymenophyllum caudiculatum during a desiccation/rehydration cycle. Garcés M, Claverol S, Ulloa M, Miranda A, Bravo L. Gordon Research Seminar on Salt & Water Stress in Plants. Les Diablerets, Switzeland May 28-29, 2016.

 

 

3. Garcés M. Introduction to two-dimensional proteomics in Symposium: Applications in Proteomics and Mass Spectrometry. Universidad de La Frontera, Temuco, Chile March 1st, 2016

 

 

4. Proteomics of Hymenophyllum caudiculatum, a filmy fern from southern Chilean forest. Its desiccation tolerance, is inducible or constitutive? Garcés M. 5th International Workshop Advances in Science and Technology of Bioresources. Universidad de La Frontera, Pucón, Chile December 2-4 2015

 

 

5. Functional characterization of Hymenophyllum caudiculatum (filmy fern) treated with actinomycin D(transcription inhibitor) and cycloheximide (translation inhibitor). Miranda A, Bravo L, Garcés M.5th International Workshop. Advances in Science and Technology of Bioresources. Universidad de La Frontera, Pucón, Chile. December 2-4 2015

 

 

6. Ultraestructural changes in Hymenophyllum caudiculatum during a dehydration/rehydration cycle. Ulloa M, Bravo L, Garcés M. 11th International Congress of Plant Molecular Biology. Foz de Iguazú. Brazil October 25-30, 2015.

 

 

7. Proteomic response of Chlorella sp CH03 exposed to temperature stress. Hayashida G, Garcés M, Traipi E, Rivas M. V Latin American Congress of Algae Biotechnology. Viña del Mar, Chile October25-29, 2015.

 

 

8. Proteomic response of Nannochloropsis gaditana CCMP-527 exposed to stress by Nitrogen deprivation. Hayashida G, Garcés M, Ulloa M, Huentecol T, Rivas M. V Latin American Congress of Algae Biotechnology. Viña del Mar, Chile October 25-29, 2015.

 

 

9. Desiccation tolerance in Hymenophyllum plicatum: A biological view through the lipid composition. Rabert C, Garcés M, Bravo L. The 5th PanAmerican Plant Membrane Biology Workshop. San Pedro de Atacama, Chile June 1-5, 2015.

 

 

10. Production of Lipids and Protein by Schizochytrium aggregatum under Different Growth Conditions. Shene C, Vergara D, Garcés M. 12th Euro Fed Lipid Congress “Oil, Fats and Lipids: From lipidomics to industrial innovation” Montpellier, France. September 14-17, 2014.

 

 

11..Transcriptional and translational inhibition of Hymenophyllum caudiculatum during a dehydration/rehydration cycle, Physiological and DIGE analysis. Garcés M, Traipi E, Huentecol T, Vergara D, Claverol S. IX Reunión de Biología Vegetal. Hotel Enjoy Coquimbo Chile, December 1 -4. 2014

 

 

12. Proteomic Analysis of Deschampsia antarctica desv. in response to cold stress. Garcés M, Rabert C, Gutiérrez A, Sandoval A. IX Reunión de Biología Vegetal. Hotel Enjoy Coquimbo, Chile. December 1- 4, 2014.

 

 

13. Desiccation tolerance of Hymenophyllacea filmy ferns is mediated by constitutive and not inducible cellular mechanisms. Garcés M, Claverol S, Alvear C, Rabert C, Bravo L. VIII Reunión de Biología Vegetal, Gran Hotel Pucón, Pucón, Chile, December 2-5, 2013.

 

 

14. Standardization of total protein extraction from bioresources to perform proteomic studies, Traipi E, Huentecol T, Hayashida G, GarcésM. 4th International Workshop Advances in Science and Technology of Bioresources. Universidad de La Frontera, Pucón, Chile. December 4-6 2013.

 

 

15. Impact of lipid levels on intestinal microbiota of rainbow tout (Oncorhynchus mykiss): microbiological and DGGE analysis. Garcés M, Spuler M, Leyton A, Rabert C, Espinoza F, Shene C. 3rd International Workshop Advances in Science and Technology of Bioresources. Universidad de La Frontera, Pucón, Chile. November 2-4, 2011.

 

 

16. HPV detection and typing kit for therapeutic decisions in preneoplastic and neoplastic cervical lesions. Garcés M, Montenegro S, Melo A, Roa JC. et al. Encuentro Binacional Sur de Investigadores Jóvenes del Bicentenario Argentina – Chile. Calafate, Santa Cruz, Argentina. October 6-7, 2010.

 

 

17. Understanding wood formation combining omics technologies. Chaumeil P, Garcés M, Le Provost G, Lalanne C, Rodrigues JC, Alve A, Claverol S, Paiva J, Herrera R, Plomion C. 17th international conference of the Plant and Animal Genome, San Diego, CA, EEUU. January 10-14, 2009.

 

 

18. Recombinant Taq Polymerase for Molecular Diagnostic Applications. Garcés M. First regional meeting on science, technology and innovation: engine of the Development of the Bio-Bio Region. Centro de eventos suractivo, Concepción, Chile. October , 2008.

 

 

19. Proteomics of wood formation in maritime Pine. Garcés M, Lalanne C, Le Provost G, Paiva J, Claverol S, Herrera R, Plomion C. IUFRO Tree Biotechnology. Ponta Delgada, Azores, Portugal, June 3-8, 2007.

 

 

20. Results from project A7- A proteomic approach to study seasonal and cambial age effect on wood formation in maritime pine. Garcés M, Herrera R, Plomion C. GEMA-meeting. Puerto Iguazú, Argentina February 19-23, 2007

 

 

21. La protéomique de la qualité du bois chez le pin maritime. Rencontres Théses des bois édition 2007. Garcés M, Herrera R, Plomion C. ENITA de Bordeaux. Bordeaux, France. January 26, 2007.

 

 

22. Effect of cambial age in protein accumulation in maritime pine wood forming tissue. Garcés M, Lalanne C, Le Provost G, Claverol S, Herrera R, Plomion C. Plant and Animal Genome XV Conference. Town &Country Convention Center, San Diego, California, EEUU January 13-17, 2007.

 

 

23. Proteomic study of the wood formation in maritime pine. Garcés M, Lalanne C, Le Provost G, Paiva J, Claverol S, Herrera R, Plomion C. XXX Annual Meeting Society of Biochemistry and Molecular Biology of Chile. Termas de Chillán, Chile. September 25-28,2007.



ACADEMIC ACTIVITIES


Undergraduate courses:


2013 to 2014(1) IIQ148 Advanced Molecular Techniques
2011 to 2012 (1-2) IIQ356 General Biochemistry
2011 (1-2) IIQ312 Fundamentals of Biochemistry
2010 to 2011 (1) APA400 Advanced biotechnological processes II
2011 (1) Bioethics and Biosecurity
2010 (2) Research Seminar


Graduate courses


2014 (2) Protein Biochemistry: structure, Function & Proteomics. Coordinator
2013 (1) DAP109 Basic two-dimensional protein methods
2010 (1) Applied Molecular Biology (lecturer)
2010 (1) Biochemistry and molecular biology techniques (Lecturer)
2010 (1) Cell and Molecular Biology


THESIS SUPERVISION


Graduate


2014-2016 Gladys Hayashida Soiza. Doctoral thesis: Effect of temperature and nitrogen on the synthesis of proteins linked to the metabolic pathways of fatty acids in Nannochloropsis gaditana CCMP-527 and Chlorella sp. CH03. Doctorate in Biological Sciences mention Cell and Molecular Biology. Universidad de Antofagasta. External evaluator.


Undergraduate


2016 Álvaro Miranda. Biochemistry UFRO
2016 Marlys Ulloa. Biotechnology UFRO
2014 Eliza Traipi Biotechnology (Co-tutor) UFRO
2014 Tatiana Huentecol . Biotechnology (Co-tutor) UFRO
2012 Catalina Vidal Pérez Biotechnology (Co-tutor) UFRO

 

Outreach activities


Poster: Genetic modification of Plants. Garcés M. XI FIUFRO. Hotel Dreams, Temuco. 07 September 2017
Poster : Resurrection ferns. X FIUFRO. Hotel Dreams, Temuco. 31 August 2016
Poster: Transcriptional and translational inhibition of Hymenophyllum caudiculatum during a desiccation/rehydration cycle, physiological and Dige analysis” VIII FIUFRO. Hotel Dreams, Temuco. October, 2014
Poster: Structural modifications of the filmy fern Hymenophyllum caudiculatum during a desiccation/ rehydration cycle. VIII FIUFRO. Hotel Dreams, Temuco. October, 2014
Participation in "Explora tu aula" program with talk: "Study of plant stress using high performance proteomics" in Liceo Agropecuario Likan Antai San Pedro de Atacama. October 29th 2014
Participation as an evaluator in the Regional Congress Explora Magallanes, Universidad de Magallanes, Punta Arenas. October 17-18, 2013
Poster: Proteomic study of the tolerance to the desiccation of film ferns, use constitutive or inducible mechanisms?. VII FIUFRO. Hotel Dreams, Temuco. 2 October, 2013
Poster: Standardization of high performance proteomic studies, M Garcés, S Claverol. VI FIUFRO. Hotel Dreams, Temuco October 10, 2012
Poster of project PIA DI10-7006 and Fondecyt 3120195 VI FIUFRO Hotel Dreams, Temuco October 10, 2012
Poster: Impact of lipid levels on intestinal microbiota of rainbow trout (Oncorhynchus mykiss): microbiological and DGGE analysis. V FIUFRO Hotel Dreams, Temuco. October 19, 2011
Human papillomavirus (HPV) detection and typing kit for therapeutic decisions in preneoplastic lesions and cervical cancer. Montenegro, Melo, Guzman, Antonio, Garcés, Ili, Roa et al. IV FIUFRO Universidad de la Frontera. Temuco. January 11-15, 2010
Participation in activity “1000 científicos 1000 aulas”, Santa cruz, Séptima región. 2008