Director
Scientific and Technological Bioresource Nucleus
Phone:56-45-2734191
Fax:56-45-2325479
mariluz@ufro.cl
BIOREN / Professional Support
The Flow cytometry is a multiparametric and quantitative method that analyzes scattered and fluorescent signals produced by a cell passing through a beam of light (laser), It is considered an analytical tool, fast and sensitive. The equipment with which BIOREN accounts is a Flow Cytometer FACs Canto II by Becton Dickinson of double laser (blue 488 nm and red 633 nm), with the possibility of adding a third laser (violet 405 nm), this equipment measure cells in suspension of 0.2 to 100 microns in size. The information provided corresponds to eight cells parameters in total: Side Scatter (SSC, cell complexity), Foward Scatter (FSC, cell size) and 6 channels of fluorescence, which allows us to characterize the cell in its structural and metabolically.
Confocal Laser Microscopy (CLSM) Olympus Fluoview 1000
This microscope is a powerful tool capable of acquiring fluorescent images in a particular optical section and focus on well defined areas with a very good spatial and temporal resolution. The researcher can perform 3D reconstructions, in vivo experiments, localize organelles, between other. CLSM is widely-used in numerous biological science disciplines, from cell biology and genetics to microbiology and developmental biology. Clinically, CLSM is used in the evaluation of various eye diseases. Research into CLSM techniques for endoscopic procedures is also showing promise. In the pharmaceutical industry, it was recommended to follow the manufacturing process of thin film pharmaceutical forms, to control the quality and uniformity of the drug distribution
Technical data: Laser lines: Multi Argon Laser (458nm, 488nm, 515nm), HeNe(G) Laser (543nm), LD Laser (405nm, 635nm); Objectives: Olympus UPLANFL 4x, Olympus UPLANFL 10x, Olympus UPLANFL 20x, Olympus UPLSAPO 40x, Olympus UPLSAPO 60x (Water immersion), Olympus UPLSAPO 100x (Oil immersion); Software: FV10-ASW 2.0 (Acquisition, 3D preview, line analysis, etc). OIB, OIF and TIFF image format.
Laboratory of Gas Chromatography and Particle Analysis
• Gas chromatograph coupled with thermal conductivity detector and flame photometric detector (GC/TCD-FPD). The TCD is a universal detector responding to most compounds, as well as gases such as oxygen, nitrogen, and carbon dioxide. The FPD, a highly sensitive and selective detector for both sulfur and phosphorus compounds. Especially suitable for environmental monitoring of H2S and sulfur gases in general and for organophosphorus compounds present at trace levels. • Gas chromatograph coupled with flame ionization detector (GC/FID). The FID is the most widely and successfully used gas chromatographic detector for volatile hydrocarbons and many carbon containing compounds. Molecules that contain only carbon and hydrogen respond best in this detector but the presence of "heteroatoms" in a molecule, such as oxygen, decreases the detector's response.
• Surface area and pore size analyzer (NOVA 1000e). Porosimetry is an analytical technique used to determine various quantifiable aspects of a material's porous nature, such as pore diameter, total pore volume, surface area, and bulk and absolute densities. The technique involves the intrusion of a non-wetting liquid (often mercury) at high pressure into a material through the use of a porosimeter. Our equipment use N2 as adsorbate and can measure BET surface area from 0.01m2/g to more than 2000 m2/g and pore size range of 3.5 to >4000 Å. Can be applied to Carbon, Catalysts, Organic Materials, Minerals, Powdered Metals and Ferrites, Bone, Composite Materials, Fibers, Rigid Foams, Soil, Sludge among others.
• Laser diffraction particle size analyser (SALD-3101). This equipment is useful wherever there is a need to measure coarse or dense particles, including the fields of metals, minerals, pharmaceuticals, chemicals, environment, river conservation, disaster prevention, civil engineering, construction, agriculture, and soil. Seamlessly covers an extremely wide range of particle diameters from 0.05 to 3000µ using a single measurement principle, a single optical system, and a single light source. Always achieves accurate particle size distribution measurements across the entire measuring range using a single standard, without data discontinuities from a multiple optical system combination.
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HPLC/MS System
High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry. Is a powerful technique used for many applications which has very high sensitivity and specificity. Generally its application is oriented towards the specific detection and potential identification of chemicals in the presence of other chemicals (in a complex mixture).
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qPCR Step One Plus, Applied BiossystemsThe identification of gene expression and specific DNA or RNA sequences is a crucial factor when working with molecular biology techniques. Real-time PCR technique (qPCR) provides large amounts of data with a high sensitivity and specificity, using modern platforms to quantify the PCR product. Biossystems Applied equipment for Real-Time PCR is the reference tool and with higher market presence, basically performs the identification of gene expression and specific DNA or RNA sequences, crucial factor when is necessary to work with molecular biology techniques. This equipment technically consist on thermocycling Peltier system with 96-well block for sensing, as well to well LED excitation source is continuous use for at least 10 years, an optical system consisting of 4 channels and a free preconfigured software to perform applications such as absolute quantification, relative quantification (curve), relative (difference method) and SNPs genotyping, determinations that are essential in the cross scientific area and a very good complement for techniques such as flow cytometry (CMF) and confocal microscopy (CLSM).
Two- Dimensional Electrophoresis (IEF - 2D), BIORADTwo-dimensional electrophoresis is the most widely used tool for global analysis and separation of the proteome components. This technique allows separation of hundreds or thousands of protein spots on a single gel showing a characteristic pattern, which is the most efficient way to isolate and purify for proteomic analysis. This technique is complementary to the analysis by flow cytometry (CMF) and confocal microscopy (CLSM).
Multimodal Detector SynergyTM HT , BIOTEKSynergyTM HT multimode microplate reader is coupled with methodologies such as fluorescence, luminescence and absorbance. It uses a unique dual perspective of design that allows the use of three reading modes without compromise. SynergyTM HT is the perfect addition to flow cytometry (CMF) and confocal microscopy (CLSM) because it makes fluorescence quantifications with a reagent dispenser system with automatic injection (intracellular calcium, ORAC antioxidant activity, FRET, among others) Technically has capacity from 6 to 384 samples, using as average volume 200 uL, this decreases the cost of reagents and determination time, the use of tungsten-halogen lamp produces a large amount of light at a constant intensity, providing greater sensitivity and repetition capacity. Specially designed with fluorescence interference filters to prevent light excitation wavelength leakage of the emission wavelength, moreover, the possibility of using different bandpass filters allows the fluorescence measurements adapt with more effectively. For absorbance measurements the instrument switches to a xenon flash lamp and a monochromator (200-999 nm whole UV / Vis spectrum in 1 nm increments) for wavelength selection. This equipment also has a control system for 4-ZoneTM that ensures uniform temperature for kinetic assays. Synergy HT counts with a plate for microvolumes (take3, nanoq) useful for DNA, RNA and proteins quantifying, with only 2 uL of sample for 16 sample determinations at the same time.
Semipreparative HPLC Prominence LC-20A, ShimadzuThe high efficiency liquid chromatography is widely used in diverse areas, as well as pharmaceutical, biochemical, chemical, environmental, food industry. The semipreparative chromatography seeks the isolation and purification of a compound of value. The advantages of this device is the possibility to obtain pure fractions of active compounds which can have different analytic interest, this thanks to the fraction collector FRC-10A, Shimadzu that the equipment possess. In addition to the versatility of a standard HPLC, is added the specificity of a detector (DAD) that could be employed in the simultaneous scanning of analytes from different families. Finally, samples that achieve purity and cleanliness criteria can be obtained, to use them later on further analysis systems ms / ms
Basic Research Development equipment
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